Coding

Part:BBa_K1809015:Design

Designed by: Edward Dring   Group: iGEM15_WPI-Worcester   (2015-08-03)


ChAFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 12


Design Notes

Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.


Source

Taken from the Atlantic herring Clupea harengus. Pubmed ID: 9521729

References